Rapid and visual detection of 2019 novel coronavirus (SARS-CoV-2) by a reverse transcription loop-mediated isothermal amplification assay
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چکیده
منابع مشابه
A reverse transcriptase-loop mediated isothermal amplification assay (RT-LAMP) for rapid detection of bovine viral diarrhea virus 1 and 2
Bovine viral diarrhea virus (BVDV) is a pathogen that infects cattle, and is globally important. It causes substantial financial losses to the livestock industry. In the current study, a one-step reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) assay was set up for rapid and efficient detection of BVDV. For this purpose, four primers were designed to recognize six distinct...
متن کاملRapid detection of the severe acute respiratory syndrome (SARS) coronavirus by a loop-mediated isothermal amplification assay.
drome (SARS) Coronavirus by a Loop-Mediated Isothermal Amplification Assay, Leo L.M. Poon, Cynthia S.W. Leung, Masato Tashiro, Kwok Hung Chan, Bonnie W.Y. Wong, Kwok Yung Yuen, Yi Guan, and Joseph S.M. Peiris ( Department of Microbiology, Queen Mary Hospital, University of Hong Kong, Hong Kong SAR; 2 Department of Viral Diseases and Vaccine Control, National Institute of Infectious Diseases, Mu...
متن کاملReverse transcription loop-mediated isothermal amplification assay for rapid detection of Bovine Rotavirus
BACKGROUND Bovine rotavirus (BRV) infection is common in young calves. This viral infection causes acute diarrhea leading to death. Rapid identification of infected calves is essential to control BRV successfully. Therefore development of simple, highly specific, and sensitive detection method for BRV is needed. RESULTS A reverse transcription loop-mediated isothermal amplification (RT-LAMP) ...
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The one-step single-tube betaine-free reverse transcription (RT)-loop-mediated isothermal amplification assay was developed for rapid diagnosis of hepatitis E virus. This assay amplified the target gene in less than 45 min (even as short as 20 min) under isothermal conditions at 63 degrees C, and the sensitivity of this assay was 100-fold greater than that of RT-PCR. This assay demonstrated a d...
متن کاملRapid detection of infectious bursal disease by loop-mediated isothermal amplification for field analysis
Infectious bursal disease (IBD) is an immunosuppressive, acute and highly contagious illness of growing-poultry stock infected with infectious bursal disease virus (IBDV). It is common in Pakistan, causing potential economic losses throughout the year. The objective of the study is to propose a rapid, sensitive and specific diagnostic tool, and compare it with existing commonly used reverse tra...
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ژورنال
عنوان ژورنال: Clinical Microbiology and Infection
سال: 2020
ISSN: 1198-743X
DOI: 10.1016/j.cmi.2020.04.001